![]() ![]() Included are steps for identifying secondary objects (fluorescent marker signals) and relating these objects to individual worms, enabling count of signals on a per-worm basis. Measurements using a low-resolution atlas: Once worms are untangled, this pipeline shows how they can be straightened and aligned with a low-resolution worm atlas to extract localized intensity measurements and compare patterns of reporter If adjusting the pipeline to fit your own data, worm detection will likely improve by creating a new worm model based Untangling requires a worm model, which is provided together with the pipeline.
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